Ovine prion protein genotype frequencies in northwestern China

Novel polymorphisms in ovine prion protein gene.

The aim of this study was to identify the PRNP polymorphisms outside the standard codons 136, 154 and 171 in 1110 sheep with no clinical sign of scrapie from all 18 Turkish native sheep breeds and compare our results with published data on ovine PRNP polymorphism from other regions of the world. Among the 22 amino acid polymorphisms and three silent mutations, 10 were novel for ovine PRNP: p.Gl...

Transmissibility of Atypical Scrapie in Ovine Transgenic Mice: Major Effects of Host Prion Protein Expression and Donor Prion Genotype

Atypical scrapie or Nor98 has been identified as a transmissible spongiform encephalopathy (TSE) that is clearly distinguishable from classical scrapie and BSE, notably regarding the biochemical features of the protease-resistant prion protein PrP(res) and the genetic factors involved in susceptibility to the disease. In this study we transmitted the disease from a series of 12 French atypical ...

Hepatitis E Virus Genotype 4 in Yak, Northwestern China

al. Identification of rodent homologs of hepatitis C virus and pegiviruses. et al. Serology-enabled discovery of genetically diverse hepaciviruses in a new host. To the Editor: Hepatitis E virus (HEV; family Hepeviridae, ge-nus Hepevirus) is a positive-stranded RNA virus with a genome of ≈7.2 kb that contains 3 open reading frames (1,2). On the basis of sequence analysis , mammalian HEVs are cl...

Allele Frequencies and Genotype Frequencies

Genotyping of Ovine Prion Protein Gene (PRNP) Vari-

Background: Scrapie is the transmissible spongiform encephalopathy in sheep. Because genetic variants of the ovine PrP gene (PRNP) can be associated with disease risk, the European Union initiated programs to eradicate high-risk PRNP genotypes from sheep livestock. For this purpose, reliable and cost-effective genotyping is needed. Methods: We amplified DNA to cover the 3 risk codons in exon 3 ...